PEMANFAATAN KACANG HIJAU VIMA 1 DAN LOKAL SEBAGAI MEDIA ALTERNATIF PENGGANTI MEDIA SABOURAUD DEXTROSE AGAR (SDA) DALAM PERTUMBUHAN JAMUR TRICHOPHYTON RUBRUM

Abstract

Mung beans are a kind of cultivated plants and crops that are used as a source of food with nutritional content in the form of vegetable protein. Varieties of mung beans are diverse including Vima 1 and local which has a high carbohydrate and protein content. The fungus Trichophyton rubrum is the cause of dermatophytosis. Therefore, research on the use of green beans Vima 1 and local as an alternative medium in the growth of Trichophyton rubrum fungus. The type of research used by experimental laboratory was conducted at STIKes Hutama Abdi Husada Tulungagung Microbiology Laboratory in March - April 2022. SDA Media and alternative media of mung bean Vima 1 and local with mass variation of 6 grams and 7 grams inoculated Trichophyton rubrum fungus using single dot method and incubated at room temperature for 14 days with 5 times repetition. The results showed that Trichophyton rubrum fungus grows optimally on alternative media of mung bean Vima 1 7 gram mass variation that grows the 5th day colony with an average final diameter of 14 mm. While Vima 1 mung bean alternative media mass variation 6 grams 11 mm, SDA media 11.65 mm, local mung bean alternative media mass variation 6 grams 7.5 mm, and mass variation 7 grams 11.5 mm. Based on statistical test-T Independent obtained P_value < 0.00 which shows there are significant differences in each type of media. The conclusion of this study shows that green beans Vima 1 and local can be used as an alternative medium to substitute for natural resources in the growth of Trichophyton rubrum fungus. So it is known that alternative media of green beans can be used to reduce the use of natural resources media.